NEET-PG 2012 - Biochemistry Practice Questions

Q: Carnitine is synthesised from -

Lysine. ***Lysine*** - **Carnitine** is synthesized in the liver and kidneys from the amino acids **lysine** and methionine. - **Lysine provides the essential carbon backbone** for carnitine synthesis (trimethyllysine is the actual precursor formed from protein-bound lysine residues). - Methionine contributes methyl groups via S-adenosylmethionine (SAM), but lysine is the primary structural precursor. *Arginine* - **Arginine** is a precursor for **nitric oxide**, urea, and creatine, but not a direct precursor for carnitine synthesis. - While arginine is an amino acid, its metabolic pathways are distinct from those involved in carnitine formation. *Histidine* - **Histidine** is a precursor for **histamine** and contributes to protein synthesis, but is not involved in carnitine biosynthesis. - Its metabolic fate differs significantly from the pathway leading to carnitine. *Choline* - **Choline** is a precursor for **acetylcholine** and phospholipids, but not directly for carnitine. - Although both choline and carnitine contain methyl groups, they have different biosynthetic origins.

Q: Which cofactor is primarily associated with the activity of glutamate dehydrogenase?

NAD+. ***NAD+*** - Glutamate dehydrogenase catalyzes the oxidative deamination of **glutamate** to **α-ketoglutarate** and ammonia, and this reaction primarily uses **NAD+** as an electron acceptor. - In some organisms and contexts, it can also use **NADP+**, but **NAD+** is the more common and significant cofactor for its catabolic role. *FAD* - **FAD (flavin adenine dinucleotide)** is typically associated with **flavoproteins** and enzymes involved in oxidation-reduction reactions, such as those in the **electron transport chain** and the **Krebs cycle**. - Enzymes like **succinate dehydrogenase** use FAD, not glutamate dehydrogenase. *FMN* - **FMN (flavin mononucleotide)** is another flavin coenzyme, similar to FAD, and is found in various **flavoproteins** and enzymes of the **electron transport chain**, such as **NADH dehydrogenase (Complex I)**. - It does not serve as a primary cofactor for **glutamate dehydrogenase** activity. *FADH2* - **FADH2** is the reduced form of **FAD**, carrying high-energy electrons to the **electron transport chain** for ATP synthesis. - It's a product or reactant of various metabolic pathways, but not a direct cofactor for **glutamate dehydrogenase**.

Q: What is the primary biochemical defect in alkaptonuria?

Deficiency of homogentisate 1,2-dioxygenase. ***Deficiency of homogentisate 1,2-dioxygenase*** - **Alkaptonuria** is an autosomal recessive disorder caused by the deficiency of **homogentisate 1,2-dioxygenase**, an enzyme in the **tyrosine degradation pathway**. - This deficiency leads to the accumulation of **homogentisic acid** in the body, which is excreted in urine and deposited in connective tissues. *Urine turns black immediately upon voiding* - While urine in alkaptonuria does **turn black**, it typically darkens upon **standing** and exposure to air, not immediately upon voiding. - The darkening is due to the oxidation of accumulated **homogentisic acid**. *FeCl3 test is negative* - The **ferric chloride (FeCl3) test** typically yields a **positive result** (transient green color) in the presence of homogentisic acid in the urine. - Therefore, a negative result would argue against a diagnosis of alkaptonuria. *Benedict's test is diagnostic for alkaptonuria* - **Benedict's test** is used to detect reducing sugars like glucose in urine and would not be used to diagnose alkaptonuria. - A positive Benedict's test in alkaptonuria is due to the reducing properties of homogentisic acid, but it is not specific or diagnostic.

Q: Which of the following metabolites is involved in glycogenolysis, glycolysis and gluconeogenesis ?

Glucose-6-phosphate. ***Glucose-6-phosphate*** - In **glycogenolysis**, **glycogen phosphorylase** breaks down glycogen into **glucose-1-phosphate**, which is then converted into **glucose-6-phosphate** by **phosphoglucomutase**. - In **glycolysis**, **glucose-6-phosphate** is isomerized to **fructose-6-phosphate** by **phosphoglucose isomerase**, committing it to the glycolytic pathway. - In **gluconeogenesis**, **glucose-6-phosphate** is the final product formed from other precursors; it can then be dephosphorylated to free glucose by **glucose-6-phosphatase**. *Galactose-1-phosphate* - This is an intermediate specifically in **galactose metabolism**, not directly involved in the central common pathways of glycogenolysis, glycolysis, or gluconeogenesis. - It is converted to **glucose-1-phosphate** via the **Leloir pathway** (involving **galactose-1-phosphate uridylyltransferase**), which can then enter glycogen metabolism. *Uridine diphosphoglucose* - **UDP-glucose** is crucial for **glycogen synthesis** (**glycogenesis**), serving as the activated glucose donor. - It is not directly a metabolite in the catabolic process of glycogenolysis, nor is it a direct intermediate in glycolysis or gluconeogenesis. *Fructose-6-phosphate* - **Fructose-6-phosphate** is a key intermediate in **glycolysis** and **gluconeogenesis**, specifically downstream from **glucose-6-phosphate**. - However, it is not directly produced from glycogenolysis; **glucose-6-phosphate** is the direct link between glycogenolysis and glycolysis.

Q: Which vitamin is considered the most potent antioxidant?

Vit E. ***Vit E*** - **Vitamin E** is a **lipid-soluble antioxidant** that primarily protects cell membranes from **oxidative damage** by scavenging free radicals. - Its ability to interrupt **lipid peroxidation** makes it highly effective in protecting tissues rich in polyunsaturated fatty acids, such as cell membranes. *Vit A* - **Vitamin A**, particularly in its carotenoid forms like **beta-carotene**, is an antioxidant, but its primary role is in **vision** and **immune function**. - While it can quench **singlet oxygen** and trap free radicals, it is generally considered less potent than vitamin E in protecting against lipid peroxidation. *Vit K* - **Vitamin K** is crucial for **blood coagulation** and **bone metabolism**, but it does not have significant antioxidant properties. - Its primary biological functions are unrelated to scavenging **free radicals** or preventing oxidative stress. *Vit C* - **Vitamin C** is a potent **water-soluble antioxidant** that works in aqueous environments, such as the cytoplasm and extracellular fluid. - While it can neutralize **reactive oxygen species** and regenerate other antioxidants like vitamin E, its solubility limits its direct activity in protecting lipid membranes, making vitamin E more potent in that specific context.

Q: In the context of cell membrane composition, what is the typical weight ratio of protein to lipid?

1 : 1. ***1 : 1*** - The **typical weight ratio of protein to lipid** in most cell membranes is approximately **1:1** (equal by weight). - While the **number of lipid molecules** far exceeds the number of protein molecules, proteins are much larger and heavier, resulting in roughly equal weight contributions. - This **1:1 ratio represents an average** for typical plasma membranes, though it can vary significantly depending on membrane type and function. *1 : 2* - This protein:lipid ratio would indicate **lipids contribute twice as much by weight** as proteins. - This is characteristic of **myelin membranes**, which are specialized for insulation and have exceptionally high lipid content. - However, this is **not typical** of most cell membranes. *2 : 1* - This ratio would suggest **proteins contribute twice as much by weight** as lipids. - While some protein-rich membranes exist, this is **higher than the average** for typical cell membranes. - The typical ratio is closer to 1:1 rather than being protein-dominant at 2:1. *4 : 1* - A 4:1 protein:lipid ratio represents an **extremely protein-rich membrane**. - This is characteristic of the **inner mitochondrial membrane**, which is packed with electron transport chain proteins. - This is a **specialized membrane**, not representative of typical cell membranes.

Q: Apoenzyme is ?

Protein moiety. ***Protein moiety*** - An **apoenzyme** is the **protein component of an enzyme** that is catalytically inactive by itself. - It requires a **non-protein cofactor** (either an inorganic ion or an organic molecule) to become active. *Organic cofactor* - An **organic cofactor** is also known as a **coenzyme**, which binds to the apoenzyme to form a functional holoenzyme. - While essential for enzyme activity, the apoenzyme itself is the protein part, not the organic cofactor. *Inactive enzyme component* - While an apoenzyme is **inactive on its own**, this description is too broad and doesn't specify its chemical nature. - It is specifically the **protein component** that is inactive until bound to its cofactor. *Non-protein component required for enzyme activity* - This describes a **cofactor** (either inorganic or organic), not the apoenzyme itself. - The apoenzyme is the **protein portion**, which *requires* the non-protein component for activity.

Question 1

Carnitine is synthesised from -

Accepted Answer

Lysine

Answer

Histidine

Answer

Choline

Answer

Arginine

Question 2

Which cofactor is primarily associated with the activity of glutamate dehydrogenase?

Accepted Answer

NAD+

Answer

FAD

Answer

FMN

Answer

FADH2

Question 3

What is the primary biochemical defect in alkaptonuria?

Accepted Answer

Deficiency of homogentisate 1,2-dioxygenase

Answer

FeCl3 test is negative

Answer

Urine turns black immediately upon voiding

Answer

Benedict's test is diagnostic for alkaptonuria

Question 4

Which of the following metabolites is involved in glycogenolysis, glycolysis and gluconeogenesis ?

Accepted Answer

Glucose-6-phosphate

Answer

Uridine diphosphoglucose

Answer

Fructose-6-phosphate

Answer

Galactose-1-phosphate

Question 5

Which one of the following statements concerning gluconeogenesis is correct?

Accepted Answer

It is important in maintaining blood glucose during the normal overnight fast.

Answer

It occurs primarily in the liver.

Answer

It is stimulated by elevated levels of acetyl CoA.

Answer

It is primarily inhibited by insulin.

Question 6

Which of the following best describes the difference between glucokinase and hexokinase?

Accepted Answer

Glucokinase has higher Km for glucose compared to hexokinase.

Answer

Glucokinase is not inhibited by glucose-6-phosphate unlike hexokinase.

Answer

Glucokinase has a low affinity for glucose.

Answer

Glucokinase activity increases with glucose concentration while hexokinase remains saturated.

Question 7

What metabolic changes occur during overnight fasting?

Accepted Answer

Glucose production increases

Answer

Blood glucose decreases slightly

Answer

Fat breakdown increases

Answer

Ketone levels rise slightly

Question 8

Which vitamin is considered the most potent antioxidant?

Accepted Answer

Vit E

Answer

Vit A

Answer

Vit K

Answer

Vit C

Question 9

In the context of cell membrane composition, what is the typical weight ratio of protein to lipid?

Accepted Answer

1 : 1

Answer

1 : 2

Answer

4 : 1

Answer

2 : 1

Question 10

Apoenzyme is ?

Accepted Answer

Protein moiety

Answer

Organic cofactor

Answer

Inactive enzyme component

Answer

Non-protein component required for enzyme activity

NEET-PG 2012 — Biochemistry