PCR Technology Practice Questions

Question 1

Steps of PCR in sequence are?

Accepted Answer

Denature DNA, Anneal Primers, Extend DNA

Answer

Denature DNA, Extend DNA, Anneal Primers

Answer

Anneal Primers, Extend DNA, Denature DNA

Answer

Extend DNA, Anneal Primers, Denature DNA

Question 2

Which of the following is a primarily RNA based technique?

Accepted Answer

Northern blotting

Answer

Western blotting

Answer

Southern blotting

Answer

Sanger's technique

Question 3

Which one of the following enzymes is obtained from Thermus aquaticus bacterium that is heat stable and used in PCR at high temperature?

Accepted Answer

Taq polymerase

Answer

DNA gyrase

Answer

DNA polymerase III

Answer

Endonuclease

Question 4

DNA amplification is done by all, except:

Accepted Answer

DNA sequencing

Answer

Loop-mediated isothermal amplification (LAMP)

Answer

Ligase chain reaction

Answer

Polymerase chain reaction

Question 5

Which among the following is the best sample for DNA in advanced burnt dead body?

Accepted Answer

Femur

Answer

Spleen

Answer

Enamel

Answer

Sternum

Question 6

DNA matching used for identification and comparison among family members is based on analysis of which genetic marker?

Accepted Answer

Short tandem repeats (STRs)

Answer

Single nucleotide polymorphisms (SNPs)

Answer

Ribosomal RNA genes

Answer

Mitochondrial DNA

Question 7

What is the underlying principle of using mitochondrial DNA rather than nuclear DNA for identification in severely degraded disaster victim remains?

Accepted Answer

Mitochondrial DNA is present in thousands of copies per cell making it more likely to be recovered

Answer

Mitochondrial DNA is more specific for individual identification

Answer

Mitochondrial DNA is not affected by environmental factors

Answer

Mitochondrial DNA analysis is less expensive and faster

Question 8

Absolute proof of monozygosity is determined by?

Accepted Answer

DNA fingerprinting

Answer

Intervening membrane layers

Answer

Sex of the babies

Answer

Reciprocal skin grafting

Question 9

What is a confirmatory test for species of origin?

Accepted Answer

Ouchterlony Test

Answer

Origin Test

Answer

Olympus Test

Answer

SOO Test

Question 10

Which of the following is the best method for paternity testing?

Accepted Answer

Microsatellite analysis

Answer

Karyotyping

Answer

Northern blot analysis

Answer

All of the above

PCR Technology — MCQs

PCR Technology — MCQs

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